| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054407 |
| Chromosome: | chromosome 10 |
| Location: | 6602030 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801215 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGCCCGGTACCTGCTTGCCCGGCACGAACAGGTCCGGCCGGTGCGG |
| Internal bar code: | ATTTGACGGGTTGGGAAGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2730 |
| LEAP-Seq percent confirming: | 90.8163 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACGCCCTATCCAAATA |
| Suggested primer 2: | GAAGGCTGGGGATATCACCG |