| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.054408 |
| Chromosome: | chromosome 17 |
| Location: | 2858036 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718750 | FAP309 | EF-Hand Containing Flagellar Associated Protein 309; (1 of 1) IPR001763//IPR002048//IPR011992 - Rhodanese-like domain // EF-hand domain // EF-hand domain pair | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTGGTTAAGCAACACAGCGACCGTGTAAAGGTGTTGCGGGAGTGAGA |
| Internal bar code: | TACATGGGCAGCGTTATCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGACCCAGAGCAAGCC |
| Suggested primer 2: | AAGGAGTTCAGGCGTGAAGG |