| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.054434 |
| Chromosome: | chromosome 12 |
| Location: | 3277014 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499250 | PDE10 | (1 of 4) K13293 - cAMP-specific phosphodiesterase 4 (PDE4); 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATGGTCGTGCACCATCTGCACGGGTGGAGCGGCCGTTGCGTGTTGCG |
| Internal bar code: | CTGTTGCTGTAGCATGGAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3189 |
| LEAP-Seq percent confirming: | 85.3659 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCTGGTGTTGCCTTGTT |
| Suggested primer 2: | GAAGGCTGTTCCGTCACTCA |