| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.054454 |
| Chromosome: | chromosome 9 |
| Location: | 4093003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399215 | FAP341 | Flagellar Associated Protein 341; (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCACCTCCAGAATGACGGCCTCTCCTCGCCCCACCGGCTGCGGCCCT |
| Internal bar code: | TTGTTCTAGCATGGCTCACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4928 |
| LEAP-Seq percent confirming: | 54.386 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAACGGTCAGCATTCACA |
| Suggested primer 2: | CTCGCAACGCCTTTTCCAAA |