Insertion junction: CLIP2.054466_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054466
Chromosome:chromosome 2
Location:5451232
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800237 (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGAAAAAGAGTTGGGACTTCCAATCAGCCCGCTCTTCGACCGCGCCAA
Internal bar code:ACCATTTGTGGCGTCACTTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1188
LEAP-Seq percent confirming:9.52381
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCCTTGCTAGACTCGATC
Suggested primer 2:CAACGGTCAACAGACGCTTG