| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.054470 |
| Chromosome: | chromosome 12 |
| Location: | 4160293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517600 | (1 of 1) K04733 - interleukin-1 receptor-associated kinase 4 (IRAK4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGAACGAGGTGCGCCTGGCCCGCAGCATCCAGCACCCGCACGTGGTG |
| Internal bar code: | AGCCTGCTAAGTACAAGACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1221 |
| LEAP-Seq percent confirming: | 26.3158 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTTGGCGGGCTTGATA |
| Suggested primer 2: | TCATTCGGCTTGTGCTCACT |