| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054484 |
| Chromosome: | chromosome 10 |
| Location: | 3935191 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446750 | (1 of 2) PTHR13690//PTHR13690:SF71 - FAMILY NOT NAMED // PROTEIN C37C3.9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCACGTGGGCCAACATGAGCCCCATGCCGTACGCTGTGCACCACGCC |
| Internal bar code: | CGTAGTTAGTGCTCCTCGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3994 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACCATCCCTCCTGTGTG |
| Suggested primer 2: | TAGTCGACACAGCGCTTGTT |