| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.054492 |
| Chromosome: | chromosome 3 |
| Location: | 5538293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185100 | (1 of 1) K15698 - RING finger protein 121 (RNF121) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAAGCCAGCCAATTGCCAAGACATGCAATCTAGGCACAAGTTGCAGG |
| Internal bar code: | TTATACAGCCTATTCTAATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3032 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAAAATTGTGGGCGTCA |
| Suggested primer 2: | ACGCACACCACTCACATCAT |