| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054494 |
| Chromosome: | chromosome 2 |
| Location: | 6222988 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCATTGGTATTCTTGGGCTTGCTTTTGCTGTCGGCATGTGCAGCCGGC |
| Internal bar code: | TAGTTAGAAGCACACCTATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 23.2877 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGCATGTGTGTGTGTG |
| Suggested primer 2: | AGGGCCAACTTCATGAAGGG |