| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054497 |
| Chromosome: | chromosome 17 |
| Location: | 1725491 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708450 | MMP33 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGCTGTTGCAAGAACGTGAGTCACCGTTCTGGTATAACTCCGCTAC |
| Internal bar code: | ATAACTGCAGCTCCGCAACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1842 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTTAAGGCAGGCAACCG |
| Suggested primer 2: | ATGTGGAGCCCTTTCGACTG |