| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054510 |
| Chromosome: | plastome |
| Location: | 148968 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802322 | icreI,341940450,I-CreI | (1 of 2) PF00961 - LAGLIDADG endonuclease (LAGLIDADG_1); DNA endonuclease I-CreI | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATATTTTGTATTCATAAGATGTTTTATTTAATTTATTGAATTATTAAAC |
| Internal bar code: | CTCTGCCATAAAAATAGGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 56 |
| LEAP-Seq percent confirming: | 4.34783 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGGGTAATTAGAGCCGTC |
| Suggested primer 2: | TGGCATGTCCTTTCAGGTCC |