| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054514 |
| Chromosome: | chromosome 9 |
| Location: | 701387 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404503 | (1 of 3) K16489 - protein SFI1 (SFI1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCCCGGCGTGCGGCTGCCTGTTGCCGCTCAAGTCGAGCCGCTGCCG |
| Internal bar code: | GTTTACCTGGTTGGTACACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4357 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCGCCTTAACTGTGTCG |
| Suggested primer 2: | TTCCTCCAGCAGTGAAGCAG |