| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054522 |
| Chromosome: | chromosome 2 |
| Location: | 3547749 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099251 | (1 of 7) PF02517 - CAAX protease self-immunity (Abi) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGGCCCGACGCCAGGTACGCCCAGCCCAACACGCCGCCCAGCGCCG |
| Internal bar code: | ATCTGGCTTACGAGTGTCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2870 |
| LEAP-Seq percent confirming: | 98.8636 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTTTCATGATGCCCGG |
| Suggested primer 2: | CGAATAAAACTGAGCCGCCG |