Insertion junction: CLIP2.054523_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054523
Chromosome:chromosome 14
Location:797777
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g612700 FAP50,DRC7 Nexin-dynein regulatory complex 7; (1 of 1) PF13415//PF13418//PF13855 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) // Leucine rich repeat (LRR_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTTGTGCCCCGCTGCGCTGTCGATACCGGCGACCACCCTTGCCAGCA
Internal bar code:ATCGATTAACCTTGTAATACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:131
LEAP-Seq percent confirming:13.5135
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCTCAATGAACATCGCCT
Suggested primer 2:TGTGTGAGGTCGTAACAGGC