| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054528 |
| Chromosome: | chromosome 14 |
| Location: | 3460745 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630400 | L-galactose dehydrogenase; (1 of 1) 1.1.1.316 - L-galactose 1-dehydrogenase / L-galDH | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGTCCCGTAGCTGTGGCGGCCCAGAACCCAACACCAGCCCAGCACCA |
| Internal bar code: | TAGTCTAGGCTAATAAAAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 41.6667 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTTGAAGTCGACGGTG |
| Suggested primer 2: | ACTCCCACCTCCCTCTCATC |