| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054537 |
| Chromosome: | chromosome 12 |
| Location: | 8386632 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550400 | GRX2 | Glutaredoxin, CPYC type, cytosolic; (1 of 2) K03676 - glutaredoxin 3 (grxC, GLRX, GLRX2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCCCTTGGGCCCAAAATACGCAATTGCCCGTTACCATCTGCCAGTCA |
| Internal bar code: | TGGTTATTCAACTTTATAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2173 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTGGGCTTTCATCTTC |
| Suggested primer 2: | GCGTTGGGAAGGACTGGTTA |