| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054539 |
| Chromosome: | chromosome 2 |
| Location: | 5833692 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117250 | (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase | 3'UTR | |
| Cre02.g800244 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCATTGCGAGATGGCATGGAGCCCCCATGCATGGGGCCCCCATGCAAA |
| Internal bar code: | ACACACTGTAAGCGGATAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2255 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCGATGTGCTATGTGC |
| Suggested primer 2: | AGAGACCTTCGCAGGGAGAT |