| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.054555 |
| Chromosome: | chromosome 10 |
| Location: | 725493 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422450 | GGT1,PGSIP6 | (1 of 1) PTHR11183//PTHR11183:SF39 - GLYCOGENIN // PROTEIN GYG-1, ISOFORM A; Plant glycogenin-like starch initiation protein 6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGTGTTAGACGGTAGACCATCTTGCGGAACATGAAGCGTTACCGAC |
| Internal bar code: | AATACATGGCAAAGTTGAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2707 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTACCTCCCTTTCCG |
| Suggested primer 2: | ACTGGCACGTCCATCAGAAG |