Insertion junction: CLIP2.054581_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054581
Chromosome:chromosome 12
Location:7253684
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g558700 FAP67 Flagellar Associated Protein 67; (1 of 4) K00940 - nucleoside-diphosphate kinase (ndk, NME) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTGACAGAGCGCAGGGCAGGCAGTCACAGTCTTCTTGGACGTACGGC
Internal bar code:CAGACCGACAGATTTTAGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:155
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGCAGTGTAGTGGGCAG
Suggested primer 2:CACACGAACACGAACCCAAC