| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054593 |
| Chromosome: | chromosome 11 |
| Location: | 1479638 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACATGAACCTTGGGATGGGCATGGGACCCAACGGCCTGGCCGGCCTG |
| Internal bar code: | TTTTCGAACGTATTAGCTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 5.55556 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCCTCACGCAGTTCTT |
| Suggested primer 2: | ATAAAGGCAGTATGGGCGGC |