Insertion junction: CLIP2.054603_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054603
Chromosome:chromosome 3
Location:544968
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145187 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCAACCCACGCCTGTACCCCGACAGCGCGGCGGCGGCGGCGCAGAAGG
Internal bar code:ATACAGTAGGCGCCGGTGGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1182
LEAP-Seq percent confirming:28.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTTGCTGCTGTATCTGC
Suggested primer 2:GCTTCCGAGTACGAAGAGGG