Insertion junction: CLIP2.054611_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054611
Chromosome:chromosome 4
Location:679816
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACTTGAGCGTGTAGAAAACAACATAACACGCCACATTGTATCATACGA
Internal bar code:CGATCAGCAAACTAGTACTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4980
LEAP-Seq percent confirming:97.561
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:AATGCTGACGAGGCTCAACA
Suggested primer 2:CCTAATCCCCACACTTGCGT