Insertion junction: CLIP2.054613_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.054613
Chromosome:chromosome 11
Location:1215733
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCTCCTGCCTGCCATCCTCCAGCACATCTCCCTCCCACCCGTGTCTC
Internal bar code:ACTGGGACTCCGAAACGGAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:159
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCACCTGCGATTTCAGT
Suggested primer 2:CCAAGCTCAAGGGGAAGGAG