Insertion junction: CLIP2.054617_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054617
Chromosome:chromosome 3
Location:8973110
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g203793 GT90-35,GT90F35 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 35 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCCAAGGGAC
Internal bar code:TGCTACTTGGTCACGATTTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2019
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGCATGGTTTGGGAAG
Suggested primer 2:ACCCAACACCCAACACACAT