| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054633 |
| Chromosome: | chromosome 12 |
| Location: | 5289940 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528150 | OST3 | (1 of 2) PF04756 - OST3 / OST6 family (OST3_OST6); Oligosaccharyl transferase complex subunit 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCATGTAGATTCAATGCTTTACGATGCAGTAGCCCATAACCCTGGCT |
| Internal bar code: | GAGTTATCAGTGGTCGGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5316 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGCTATGAAACCCACCT |
| Suggested primer 2: | TGCCTCCTGATAGCCTGACT |