| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054634 |
| Chromosome: | chromosome 16 |
| Location: | 3717212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681657 | (1 of 1) PF01344//PF13415//PF13418 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAAATCAGGTGGGCTTAGCCGCCCATCATCACGACCGCGTTCAAATCT |
| Internal bar code: | TATGATTTATACAGAAATTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2329 |
| LEAP-Seq percent confirming: | 25.4237 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGTAAAGCCTGTGGAGG |
| Suggested primer 2: | GCCACTTGCGCTTGAAGAAA |