Insertion junction: CLIP2.054637_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.054637
Chromosome:chromosome 13
Location:3842584
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACCTGGCGGCGCACACCGAGGTGACGGTGGAGTTCAGCTACCTCCG
Internal bar code:TTTAGGAGGGCGTGGTGACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1387
LEAP-Seq percent confirming:86.2069
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCAAGCAGCTAGGAACT
Suggested primer 2:TCAGCAGTCTTCCGTTTCCC