| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054641 |
| Chromosome: | chromosome 3 |
| Location: | 6051544 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190050 | (1 of 1) PF00659 - POLO box duplicated region (POLO_box) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTCCGCCACGTGATCTACATGCGGATGCTGGTGGCTGCGCCCTCGCT |
| Internal bar code: | TGTTAACGCTTATCGGCTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4508 |
| LEAP-Seq percent confirming: | 94.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTGTACGGGTCAGCAA |
| Suggested primer 2: | CATCGTCGCCGCCATAAAAG |