| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.054661 |
| Chromosome: | chromosome 3 |
| Location: | 5267033 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182300 | PGM7 | (1 of 2) 5.4.2.11 - Phosphoglycerate mutase (2,3-diphosphoglycerate-dependent) / Phosphoglyceromutase; Phosphoglycerate mutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGCAGCCCTTGAGTGCGCGCATGCTCTCGTCCGTCAGGCGGTACAG |
| Internal bar code: | TGCATACGTATCAGAATGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1176 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTTCTTCTACCGGTGA |
| Suggested primer 2: | GAGAGGCACCGCTATACACC |