Insertion junction: CLIP2.054664_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054664
Chromosome:chromosome 10
Location:4535744
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g451400 NIT4,CNX1B,CNX1E (1 of 1) 2.10.1.1//2.7.7.75 - Molybdopterin molybdotransferase // Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTCGATCCCTGGTAGGATCGTTTTGCGTTTTGGCCCCACACTGCACAC
Internal bar code:GTGTGGTAGGCTGGAATGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3932
LEAP-Seq percent confirming:55.7377
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGACCATGACCCTATTCA
Suggested primer 2:CCCCATTGCACCAACAACTG