Insertion junction: CLIP2.054664_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.054664
Chromosome:chromosome 10
Location:4535751
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g451400 NIT4,CNX1B,CNX1E (1 of 1) 2.10.1.1//2.7.7.75 - Molybdopterin molybdotransferase // Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGGTTGTCGACGTGTGTCCGTAGACCGGCGCTTTATCCATTAAGCCAT
Internal bar code:ACATTTTTGTACTCTATAACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2752
LEAP-Seq percent confirming:73.6842
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCATTGCACCAACAACTG
Suggested primer 2:CGCGACCATGACCCTATTCA