Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.054668 |
Chromosome: | chromosome 4 |
Location: | 3289762 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g226850 | ASP1 | (1 of 1) 3.4.23.5 - Cathepsin D; Pepsin-type aspartyl protease | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAAGTCGGAAGCGAAGGGTTTCGGCAGTGTTATCAGGTCTCGGGTGCG |
Internal bar code: | CTGCTCCTTTTTAATTTGTTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 699 |
LEAP-Seq percent confirming: | 47.1698 |
LEAP-Seq n confirming: | 25 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACGTTGAAACCATGCAGC |
Suggested primer 2: | TCCTGTCATTCGCATGCTGT |