| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054669 |
| Chromosome: | chromosome 15 |
| Location: | 646898 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCAATCACCGCCCCAACTCCGCGCCCCACCCCACACCTCCACTTCTT |
| Internal bar code: | GGGTTGAAACACGTGCTGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1820 |
| LEAP-Seq percent confirming: | 60.4167 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACACACACA |
| Suggested primer 2: | GAGTGAGATCTTGGGAGGCG |