| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054671 |
| Chromosome: | chromosome 9 |
| Location: | 2505298 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392950 | TMG12 | tRNA (guanosine-2'-O)-methyltransferase; (1 of 1) PTHR12029//PTHR12029:SF55 - RNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGTAGCGGCCCGCACGCACCACTCCAGCCGCAGCGCGCGCTTGCGC |
| Internal bar code: | ATTTTCCTGTTTACCAATCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1805 |
| LEAP-Seq percent confirming: | 86.8421 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACAGAGGGAGGGATAGCT |
| Suggested primer 2: | CCTTAGCTCTGCAACCCCAA |