| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054680 |
| Chromosome: | chromosome 7 |
| Location: | 2274808 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327750 | TRP3 | Transient receptor potential ion channel protein; (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATGCCTTCCCTTCTACAGTCCCCGCGCCATGTCAGTCCCCGCCCCT |
| Internal bar code: | GGCCTGAGTGATGGACCCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 16.3934 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGGATCCGGATTCTGGC |
| Suggested primer 2: | TGTGTGGTCGACTCTGCTTC |