| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054703 |
| Chromosome: | chromosome 3 |
| Location: | 6480692 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193600 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGATGGTATGGGGTGAAGAAGAAGACTCACACCTTGCTCCCAATGTTG |
| Internal bar code: | GTAGTCGACAAGGAAGTTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1270 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGGGAAAGCATTGGGGC |
| Suggested primer 2: | GGGTTCATTGCTGCCTAGGT |