Insertion junction: CLIP2.054718_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.054718
Chromosome:chromosome 7
Location:2204989
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g327200 (1 of 3) PF00534//PF13579 - Glycosyl transferases group 1 (Glycos_transf_1) // Glycosyl transferase 4-like domain (Glyco_trans_4_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGCTGCAGGCGACGGCCGGAAGCGGCCGAACCTAACTGACGCTGACAC
Internal bar code:CTACAATTTGGTACGCTTCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1564
LEAP-Seq percent confirming:91.1765
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTGCAGAAACATGCCAC
Suggested primer 2:AGCTGTCAACAGGAGAACGG