Insertion junction: CLIP2.054723_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054723
Chromosome:chromosome 16
Location:1483616
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652850 ALG5,GTR4 Dolichyl-phosphate beta-glucosyltransferase; (1 of 1) 2.4.1.117 - Dolichyl-phosphate beta-glucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGGCGCGGTCTCTCTTGCGCGGCTGCCGCTCCTCTCCCACCACCATC
Internal bar code:TTGATCGTGTATTTGAAAGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4941
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCTCTGCCATTCACAGCT
Suggested primer 2:TCGCCGTACATCACCGTATG