Insertion junction: CLIP2.054726_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.054726
Chromosome:chromosome 17
Location:1691059
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g708050 (1 of 1) PTHR23257:SF297 - PROTO-ONCOGENE SERINE/THREONINE-PROTEIN KINASE MOS CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCCGGTTCCACGGACCCCAAGTCCCGCCAGACGCTAGCGCAGGTGGTG
Internal bar code:CTTTGATAGATCTAGACAACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TCCATATCTAACTCGCCGCG
Suggested primer 2:CTGTGACGGAGGCTATGGAC