Insertion junction: CLIP2.054730_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.054730
Chromosome:chromosome 4
Location:2748304
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g223550 (1 of 26) IPR011990 - Tetratricopeptide-like helical domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCACCGCCACCGAGATGCCCACCTAGGCAGGGCACGTGCGTGTGCCGTG
Internal bar code:AATTCTCCGGGGTACGACATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:351
LEAP-Seq percent confirming:74.0741
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGACTCTGCAGGTGAGC
Suggested primer 2:ATGCAACCGCTGACAACAAC