Insertion junction: CLIP2.054749_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054749
Chromosome:chromosome 6
Location:1145869
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g257900 (1 of 1) K10088 - protein OS-9 (OS9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACATCGATTGCTGTGCTGTACCATTGCGCAAACCGCTTGCCGCTGCTC
Internal bar code:AGCCCAGCATGCTTTTGATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2609
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCTCCTGCTCAGTCAAA
Suggested primer 2:GCTGCTTTCGATCGACGTTC