| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054785 |
| Chromosome: | chromosome 5 |
| Location: | 281114 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243353 | (1 of 14) PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGGGACGAGACACACACATGGTTCGCAAGGCATGTGCGGTGGGTGCC |
| Internal bar code: | GGGCTACCTTGTTTAGAACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 4.16667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTATGTGTAGCGTGTGT |
| Suggested primer 2: | AGTAGCAGGCAGGCATTGTT |