| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054790 |
| Chromosome: | chromosome 5 |
| Location: | 1053956 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247650 | (1 of 32) IPR000536 - Nuclear hormone receptor, ligand-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGGCGTGGCAGCGCCGCCGGCACTGCCGCCGCCTCCAGGAACTCCA |
| Internal bar code: | TCTCTTTAATGTAGGCCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGGTGGGCTTTCATGAT |
| Suggested primer 2: | GTGATGAGTGGGAGGGTTGG |