| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054790 |
| Chromosome: | chromosome 10 |
| Location: | 734514 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422500 | (1 of 1) PF09113 - Peptide-N-glycosidase F, C terminal (N-glycanase_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCTGCAGTCACAGGCATGGCTACGGCCACAACCATATGCTTGGAAT |
| Internal bar code: | TTAGCGGCTATGGGTGAGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4095 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACTGGCTTCATGTCGGT |
| Suggested primer 2: | ATGCTACAGCCTCAGATCGC |