| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054803 |
| Chromosome: | chromosome 3 |
| Location: | 2775092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161750 | lunapark domain protein involved in vesicle localization | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACCGACACAGCCGCGGACGCGGCAGCCGCCGCGGGCCGCATGAGCAT |
| Internal bar code: | AGACGTGAGGGCTTCATGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4509 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGACCCGGATGAGCAGG |
| Suggested primer 2: | CCCATTCCACATTCAGGGCT |