| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054813 |
| Chromosome: | chromosome 10 |
| Location: | 719492 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422400 | (1 of 89) 3.6.4.13 - RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGTTCCAGGAAGTTGGAGGCGAAGGACGCCACTGTAACAAATCGAAG |
| Internal bar code: | GTCGTAGGATCGCTTTGTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2466 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCAGGCTTCATGTCTCA |
| Suggested primer 2: | CCGTGAATCGCGTCAGAAAC |