| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054816 |
| Chromosome: | chromosome 17 |
| Location: | 1745269 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708600 | HTB27,HTB20 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCACCCTGACACCGGCATCAGCAGCAAGGCCATGTCTATCATGAACA |
| Internal bar code: | CTTTCTTTGGTTGGACCAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3820 |
| LEAP-Seq percent confirming: | 89.3617 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGCCATGGTACCATCAC |
| Suggested primer 2: | GTTGGGTCAAACAACCGCTC |