| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054819 |
| Chromosome: | chromosome 12 |
| Location: | 3010959 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501850 | FFT1 | putative fructan fructosyltransferase; (1 of 2) PTHR31953//PTHR31953:SF1 - FAMILY NOT NAMED // BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV2-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGATATGGAAGGGGCAGTGGGGAGGGAGAAGGATGGCGGGCAGGGACA |
| Internal bar code: | CGGGCTATGTGGGGGGCCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2272 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGATCTGGGCAATGTGCT |
| Suggested primer 2: | GGATGCCCCCTGTAGATTCG |