| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054827 |
| Chromosome: | chromosome 7 |
| Location: | 423742 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315050 | GGH1 | (1 of 2) 3.4.19.9 - Gamma-glutamyl hydrolase / Pteroyl-poly-alpha-glutamate hydrolase; Gamma-glutamyl hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGGGGGGAGGGGAACGAAAGCAGCGGGAGGCGGGTTGCGTCCAGGC |
| Internal bar code: | ATCTGTGAAAGCTTCGTATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCGAGGCTAGTGTACAG |
| Suggested primer 2: | AGCGCGACTGTTTCCTTACA |