| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054852 |
| Chromosome: | chromosome 1 |
| Location: | 6626564 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047218 | (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACAGCATATGTTGCCGGTACGTGGTACGTGCAGACGTGCCGCCTTCTT |
| Internal bar code: | TTATCTTTCTGTGGCTCACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2286 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGCTGGCATGAGCATAG |
| Suggested primer 2: | GAGGAGAAAGAGGTGGTGGC |